FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68112061
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTGACCTGTGTAGATCTC3769090.5533660183913683Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG633700.091.96789
GAGCGTC704900.082.710458
AGAGCGT753900.077.400947
ATCGGAA763100.077.114251
TCGGAAG779900.075.451142
CGGAAGA819000.071.724933
AAGAGCG829600.070.6536256
GAAGAGC1284800.045.9987835
GGAAGAG1796700.033.1336864
CGCCGTA492500.025.02482855-59
TCGCCGT514650.023.95060355-59
GCCGTAT523450.023.53964655-59
GTCGCCG556250.022.0734155-59
TGGTCGC567700.022.00026350-54
CGTATCA555700.021.88342360-64
CCGTATC550350.021.35378855-59
GTGGTCG585150.021.3466650-54
GGTCGCC591250.020.1505350-54
CGTCGTG621250.019.91466310-14
TCTCGGT631250.019.89738345-49
Produced by FastQC (version 0.11.5)