FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682102
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT92280.34405850336788085No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT52300.19499631259362993No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT49320.18388562403666975Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT40470.15088911607388533No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT30060.11207627450410163Illumina PCR Primer Index 10 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27130.10115200689608374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)