FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830138
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT129770.7090722120408406No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT88620.4842257796953017Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT72110.39401400331559694No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT68570.37467119965816786No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT52090.28462334534335665Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT39860.21779778355511986RNA PCR Primer, Index 38 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27470.150097970754118No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT24580.13430681183604734Illumina PCR Primer Index 10 (96% over 27bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCTGCT19400.10600293529777537RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)