FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687340
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT74921.0899991270695726No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT72091.0488259085750866Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT55160.8025140396310413No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT42240.6145430209212326Illumina PCR Primer Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT39340.5723513835947275No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT32720.4760380597666366RNA PCR Primer, Index 38 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT24970.3632845462216661Illumina PCR Primer Index 10 (96% over 27bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCTGCT19380.2819565280647132RNA PCR Primer, Index 38 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG10150.14767073064276778Illumina PCR Primer Index 10 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.13108505252131405No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCTG8480.12337416707888381RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)