FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773025
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT130740.3465124137793945No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT104240.2762769925987768Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT82360.21828638824285554No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT71350.18910555853724798No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT62300.165119499605754Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT46260.12260719184208958RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)