FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7667289
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA209900.27376038649384415No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC178490.23279414666644233No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT171710.22395138620704136No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT147370.19220613700618303No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC147340.1921670097475131No Hit
TATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTG142820.18627183610791248No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT139240.18160264990663585No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT130700.17046442360526648No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT127300.16603000095600937No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG125260.1633693473664551No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA114580.14944004327996507No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC112360.14654462613839128No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT112080.1461794383908054No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTG110500.14411873610085651No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT106750.13922782876711703No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC101280.13209362526963572No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAAT95680.1247898703179181No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA95120.12405949482274634No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAA93460.12189445317634434No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC91440.11925988442590334No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT88270.1151254374264489Illumina PCR Primer Index 10 (95% over 22bp)
GTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAAA87380.11396466208590808No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA85000.11086056623142808No Hit
CGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATG82620.10775647037694809No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA80200.10460020484424155No Hit
CCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTTCTAGCATTAAACAAA78010.1017439149613377No Hit
GTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCA77760.10141785447242174No Hit
GGTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAA77300.10081790317281636No Hit
GTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCG76980.10040054574700394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)