FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253291
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT62130.49573482934131025No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT53380.4259186414009197Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT26520.21160289190618936Illumina PCR Primer Index 11 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT20250.1615746063763324No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT17760.1417069140367241No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT14810.11816888495967816Illumina PCR Primer Index 11 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)