FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390506
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT89860.6462395703434577No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT68590.4932736715986842Illumina PCR Primer Index 11 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT50120.360444327460651No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT47870.34426316750880614No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT35180.2530014253804011Illumina PCR Primer Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT23990.1725271232198926TruSeq Adapter, Index 18 (95% over 21bp)
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT17320.12455897349597916Illumina PCR Primer Index 11 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.1106791340706189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)