FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63920675
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTAGGAGGTGTAGATCTC2097200.32809415732859515Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG468850.071.310159
GAGCGTC527800.063.4553078
AGAGCGT580200.057.9631087
ATCGGAA588350.057.2404331
TCGGAAG606150.055.771762
CGGAAGA654950.051.7070583
AAGAGCG669450.050.46746
GAAGAGC1081400.031.577585
CGCCGTA307550.022.60054855-59
GGAAGAG1574250.022.068844
TCGCCGT325000.021.373755-59
GCCGTAT336750.020.73116955-59
GTCGCCG343200.020.05873955-59
TGGTCGC362400.019.26624550-54
CCGTATC363250.018.74817855-59
CGTATCA373250.018.66046360-64
GTGGTCG376250.018.56859450-54
GGTCGCC368050.018.41553950-54
TCTCGGT429900.016.44970145-49
TCGGTGG432550.015.80306545-49
Produced by FastQC (version 0.11.5)