FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879636
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT157170.8361725355334757No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT111490.5931467582021199Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT81170.43183893051633404No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT78090.41545277915511303No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT58190.3095812167887825Illumina PCR Primer Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT38850.20668895466994675TruSeq Adapter, Index 18 (95% over 21bp)
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT29890.1590201507100311Illumina PCR Primer Index 11 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.1277906999014703No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCTGCT19810.105392746255126TruSeq Adapter, Index 3 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)