FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749703
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT61900.8256602948100782No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT53230.7100144990749669Illumina PCR Primer Index 11 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT38300.5108689707790952No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT32620.4351056351648586No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT28790.3840187380869491Illumina PCR Primer Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT18580.24783147459727387TruSeq Adapter, Index 18 (95% over 21bp)
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCT13940.1859402990250806Illumina PCR Primer Index 11 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.1770034266903027No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTG11390.1519268296912244Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCTGCT9740.12991811423990568TruSeq Adapter, Index 3 (95% over 24bp)
TCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCTGCTTG8560.11417854803835652TruSeq Adapter, Index 3 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.11271130034160193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)