FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005111339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50554265
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA162300.021.559641
ATCGGAA190500.018.3831962
TCGGAAG207350.016.849013
CGGAAGA230150.015.368814
TATGCCG92800.011.54879545-49
CTCGTAT104200.010.242290540-44
CGTCAAC104000.010.21844830-34
CGTATGC107800.09.91491345-49
TCTCGTA102400.09.601006540-44
ATGCCGT120250.08.88837945-49
GCCGTCT127750.08.230679550-54
ACGTCAA134900.08.03903630-34
ATCTCGT133550.07.37246840-44
AGAGCAC533800.07.26488168
GTCACGT156250.07.089515725-29
AGTCACG159700.07.008993625-29
GAGCACA578200.06.8699449
CGTCTTC160100.06.794046450-54
GAAGAGC578150.06.7828166
CCGTCTT160550.06.69370450-54
Produced by FastQC (version 0.11.5)