FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538938
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA54080.2130024443290856No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT52670.20744894125023927No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT40610.15994876598010663Illumina PCR Primer Index 11 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.15258348175496997No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT38150.15025967550211938No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT29200.11500871624277552No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT28370.11173963287012129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)