FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69580711
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAAGGATCTCGTATG1038720.1492827516522503TruSeq Adapter, Index 9 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA492800.037.4117661
TCGGAAG509550.036.221742
CGGAAGA527350.035.003443
AGAGCAC921000.020.5205487
AGCACAC962850.019.7114529
GAGCACA985050.019.156828
GAAGAGC1030900.018.5799185
TATGCCG217050.018.55764245-49
CTCGTAT234650.017.22728540-44
CGTATGC241350.016.65314745-49
TCTCGTA221800.015.88508140-44
ATGCCGT255300.015.50465945-49
CGATCAA265350.014.99908930-34
TCGTATG252950.014.84027940-44
GCCGTCT270200.014.4884950-54
TCCGATC48300.014.260273
AAGAGCA1380000.014.0846246
GTCACGA289750.013.97616725-29
TGCCGTC266800.013.8200945-49
ATCTCGT262150.013.34050140-44
Produced by FastQC (version 0.11.5)