FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005111364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26489609
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTAGGAGGTGTAGATCTC695420.2625255812571639Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG194400.053.7700429
GAGCGTC223350.046.962538
AGAGCGT241550.043.542127
ATCGGAA248400.042.328981
TCGGAAG259650.040.7110822
CGGAAGA275050.038.55383
AAGAGCG275200.038.4287346
GAAGAGC448550.024.0620755
CGCCGTA116000.020.83541955-59
TCGCCGT124050.019.45996755-59
GCCGTAT130150.018.65929655-59
GTCGCCG130900.018.59669155-59
TGGTCGC138900.017.55613350-54
CGTATCA142950.017.06101460-64
GTGGTCG147000.016.63806750-54
GGAAGAG665250.016.4631614
CCGTATC139250.015.32629755-59
GGTCGCC144100.014.73936550-54
TCTCGGT173800.014.323400545-49
CTCGGTG183350.013.55362745-49
Produced by FastQC (version 0.11.5)