FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543875
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT105090.4131099208884085No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT83370.32772836715640513RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT51610.20287946538253648RNA PCR Primer, Index 47 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31490.12378752886836027No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT28640.11258414819910569RNA PCR Primer, Index 47 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT27720.1089676182988551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)