FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5491132
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT242240.4411476540720566No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT173500.3159639943093701RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT108290.1972088815202403RNA PCR Primer, Index 47 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT106740.19438614842986837No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT96560.1758471659395549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT77630.14137339987456138No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC62530.11387451621997069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58430.10640793191640631No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT55520.10110847817899843RNA PCR Primer, Index 47 (96% over 27bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55170.10047108683601123No Hit

[FAIL]Adapter Content

Adapter graph