FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262979
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT115550.5106101293913907No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT74630.3297865335913413TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT61570.27207499495134513No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT57040.2520571335394628No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT40760.18011656316740016RNA PCR Primer, Index 7 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32860.1452068269303427No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT30180.13336403033346753Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)