FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4971290
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT157320.3164570966489583No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT99910.20097399266588756TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT85980.17295309668114311No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT74450.14975992146907544No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT60790.12228214407125715RNA PCR Primer, Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)