FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469810
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT77231.6438560269044933No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT58151.2377344032694069TruSeq Adapter, Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT41100.8748217364466485No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT40670.8656691002745791No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT33580.7147570294374321RNA PCR Primer, Index 7 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT24550.5225516698239714Illumina PCR Primer Index 7 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT15620.3324748302505268TruSeq Adapter, Index 7 (96% over 26bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTGCT10390.22115323215768076Illumina PCR Primer Index 7 (95% over 24bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG10340.22008897213767267TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTGCTTG7540.16049041101721973Illumina PCR Primer Index 7 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC7340.15623337093718737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.1555948149251825No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG6890.14665503075711456TruSeq Adapter, Index 7 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCT5540.11792001021689619No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTG5460.11621719418488324Illumina PCR Primer Index 7 (95% over 22bp)
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGA5390.11472723015687192RNA PCR Primer, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTC5070.10791596602882016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)