FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105084
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT33640.30441124837568906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.2552747121485787No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT25820.23364739693996112TruSeq Adapter, Index 7 (95% over 21bp)
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA21600.19546025460507982No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT19740.1786289549029757No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT18540.16777005186936017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.16197863691809852No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT16250.14704764524687716RNA PCR Primer, Index 7 (95% over 23bp)
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14650.13256910786872309No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11640.10533135942607079No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT11080.10026387134371686Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)