FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687746
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT92760.5496087681440217No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT59340.35159318997052874TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT49420.2928165731099348No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT46480.27539689029036357No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT34820.2063106652304316RNA PCR Primer, Index 7 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT25110.14877831142837844Illumina PCR Primer Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.10368858821173327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)