FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433546
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT85910.1937726596273051No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT66830.1507371300534606TruSeq Adapter, Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT50700.11435541663490127No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT46620.10515285056250684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45010.1015214458133512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)