FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111772
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT107980.5113241391589622No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT78110.36987894526492443Illumina Paired End PCR Primer 2 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT42650.20196309071244434Illumina Paired End PCR Primer 2 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.14547024962922134No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT26200.12406642383742184No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTT22190.10507763148673246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)