FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT49870.2044122216128522No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA40690.1667843051419081No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT35650.146125841197076Illumina Paired End PCR Primer 2 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.1251394651261355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)