FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883583
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT165060.5724128627474916No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT115670.4011328961226363Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT80120.2778487735570643No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTT67620.2344999259601683No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT62080.215287716705224Illumina Paired End PCR Primer 2 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTTCT34660.12019768461667309RNA PCR Primer, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.12009364738244052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)