FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970454
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT85680.28844075686746873No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA68430.23036882577545387No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT58260.19613163509685727Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT41500.13970928349673148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.13024945008406122No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT36500.12287683970194455No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTT32960.11095946949523541No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT31570.10628005012028464No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT31440.10584240658162017No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT30910.10405816753937278No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT30900.1040245026517832Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC29750.1001530405789822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)