FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154053
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT35370.30648505744536864No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT24320.2107355554727556Illumina Paired End PCR Primer 2 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.14834673970779505No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT15650.13560902315578227No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT14260.12356451566782461Illumina Paired End PCR Primer 2 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTT14050.12174484187467993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)