FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982742
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT43750.4451829676558039No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT28970.2947874416683117RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT22990.23393728974644415No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT20640.21002460462664668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.18478909011724337No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT17650.17959952866571288RNA PCR Primer, Index 41 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT12310.12526176758498161Illumina PCR Primer Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.1044017656719668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)