FastQCFastQC Report
Mon 25 Jul 2022
EGAF00005111469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237847
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT114194.800985507490109No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT76093.199115397713656RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT57392.412895685041224No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT52852.222016674584922No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT42581.7902264901386185RNA PCR Primer, Index 41 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT27201.1435923093417197Illumina PCR Primer Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG23540.9897118735994147RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT21180.8904884232300597RNA PCR Primer, Index 41 (96% over 27bp)
TCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTG17030.7160065083856428Illumina PCR Primer Index 11 (96% over 26bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCT13540.5692735245767238Illumina PCR Primer Index 11 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG8490.3569521583202647TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC7720.3245784054455175No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA7030.29556815936295183RNA PCR Primer, Index 41 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT6940.2917842142217476RNA PCR Primer, Index 41 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTG6600.2774893103549761RNA PCR Primer, Index 41 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.2535243244606827No Hit
CTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTGA5150.21652574974668587Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTC5100.21442355800157242No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCT4350.18289068182487062No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC4220.17742498328757564RNA PCR Primer, Index 17 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1311767648950796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)