FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731234
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT76540.2802396279483926No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT49510.1812733731346344RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT39780.14564845048062525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.14517247515225717No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT33770.12364374491530203No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT33240.12170323011503226RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)