FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100879
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT63860.30396800577282174No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT46430.22100273266570802RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT35740.17011926912497102No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT33870.161218232939641No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT31060.14784287909965305RNA PCR Primer, Index 41 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.1388942437903373No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT21260.10119573759364534Illumina PCR Primer Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)