FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657991
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT117340.441461238958296No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT81300.30587011016967325RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT61700.23213020661093284No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT56770.2135823635219231No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT48600.18284486290585633RNA PCR Primer, Index 41 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT31910.12005307768160238Illumina PCR Primer Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.11726902009826219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)