FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3982762
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT205990.5172038901646646No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT156340.3925416582763419RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT109480.2748846152494174No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT105490.2648664419315038No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT92760.2329036984886368RNA PCR Primer, Index 41 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT62490.15690116557303702Illumina PCR Primer Index 11 (95% over 21bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT54790.13756784864373017RNA PCR Primer, Index 41 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.10482675088292999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)