FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843121
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT100560.5455963010567402No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT54790.29726751526351225TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT54020.29308981884531726No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT41550.2254328391896137No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT28170.1528385819487706RNA PCR Primer, Index 6 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.14247572459974142No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT20070.10889138586126466RNA PCR Primer, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)