FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420877
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT94470.39023048258957393No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT60850.25135519070155155TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT48700.20116676725005028No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT45640.1885267198622648No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT35240.1455670816815559RNA PCR Primer, Index 6 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34080.14077542973063067No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT24220.10004638814776629RNA PCR Primer, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)