FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498073
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT90900.6067795094097551No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT52220.3485811439095425No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT52100.347780114854216TruSeq Adapter, Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT40590.27094807796415793No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT29290.1955178419209211RNA PCR Primer, Index 6 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT20410.1362416918267668RNA PCR Primer, Index 2 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.13584117729910358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)