FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169632
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT117820.3717150760719225No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT75620.2385765918567203TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT62990.198729694803687No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT55520.17516229013336562No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT43130.13607257877255152RNA PCR Primer, Index 6 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35360.1115586919869562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)