FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535089
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT64040.41717450910012377No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT45250.2947711826480419TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT33100.21562267725193784No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT31920.20793582652211046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.17718842360280088No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT27030.17608099595528337RNA PCR Primer, Index 6 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.10983076551261849No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT16820.1095701943014379RNA PCR Primer, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)