FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160082
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT116640.36910434602646386No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT85720.2712587837910535TruSeq Adapter, Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTT62400.197463230384528No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGT62010.19622908519462468No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT48840.15455295147404402RNA PCR Primer, Index 6 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.12018675464750597No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCT34180.10816175023306357RNA PCR Primer, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)