FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203117
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT81530.6776564540273307No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT41860.3479295862330929RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT35830.29780977244939605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26900.22358590228548011No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTT25130.20887411614996712No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT19770.1643231705644588RNA PCR Primer, Index 29 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.1244268013834066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)