FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545041
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT58770.16578087531286662RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT42740.12056278051509137No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT36760.1036941462736256RNA PCR Primer, Index 29 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)