FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213917
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT168600.5245935100377515No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT137580.4280757717140798RNA PCR Primer, Index 29 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTT84740.2636658009525448No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT77870.24229001557912042No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT71940.2238390101548982RNA PCR Primer, Index 29 (96% over 25bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT44550.1386159007839966RNA PCR Primer, Index 29 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.13450876298298928No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCT42100.13099280410788455Illumina PCR Primer Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)