FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247961
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT111570.8940183226879685No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT67410.5401611108039434RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT45090.3613093678408219No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTT38380.3075416619589875No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT31070.24896611352438097RNA PCR Primer, Index 29 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.16891553502072582No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT17550.1406293946685834RNA PCR Primer, Index 29 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCT17170.1375844277184944Illumina PCR Primer Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)