FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455005
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT303510.878464720022113No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT225530.6527631653210343No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTT144940.4195073523772035No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT136210.39423966101351515No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT118200.34211238478670797Illumina PCR Primer Index 7 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTTCT73210.2118954965332901RNA PCR Primer, Index 7 (95% over 21bp)
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT57780.1672356479947207Illumina PCR Primer Index 7 (96% over 25bp)
CATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTTCTGCT38450.11128782736928022TruSeq Adapter, Index 7 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.10593327650755932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)