FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966057
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT87150.4432730078527733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86550.440221214339157No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA63310.32201507891175074No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT61850.3145890480286177No Hit
ATCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTAAAGGTCTCCTGCA57160.29073419539718326No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC53450.2718639388379889No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51770.26331891699986315No Hit
ATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTAGA49310.2508065635940362No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45540.23163112768348018No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44910.22842674449418304No Hit
GAGTTATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCA43770.22262833681831198No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37790.19221212813260247No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA37580.19114400040283674No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT37450.19048277847488654No Hit
GTCTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGG36810.18722753206036244No Hit
CTCCTATCTTTGGTTTTGCAAACTACGCGCAGAACTTCCAGGGCAGAGTC36730.18682062625854692No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATAAGTAA36570.1860068146549159No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35160.17883509989791752No Hit
GGTATCAGCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCA34330.17461345220408156No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34020.17303669222204646No Hit
ACTTACTACTGTCAACAGAGTTATGGTATCCCGAACACTTTCGGCCAGGG33320.16947626645616073No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32120.16337267942892805No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31880.16215196202348153No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG31640.16093124461803499No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30960.15747254530260313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30140.15330176083399413No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT29790.15152154795105127No Hit
TTCAGATACTTTGCGTTCAACTGGGTGCGACAGGTCCCTGGACAAGGGCT29470.1498939247437892No Hit
CTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTT29310.1490801131401582No Hit
CTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGGCC29280.14892752346447738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA29250.14877493378879655No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG28300.14394292739223735No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27970.14226444095974838No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC27960.14221357773452145No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT27760.14119631322998266No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27750.1411454500047557No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC27470.13972127969840142No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27370.13921264744613204No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27190.13829710939204712No Hit
CCATAACTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGTTGCAGACT27160.1381445197163663No Hit
ATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCAAACGA26320.13387200879730343No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG26220.13336337654503402No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC26140.13295647074321854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC26090.13270215461708385No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT25900.131735753337772No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG25770.13107453140982178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25700.13071848883323323No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG25630.13036244625664464No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25510.12975208755392137No Hit
CAATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTA25480.12959949787824054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC25410.129243455301652No Hit
GTCTACTTGGAATTGAGCAGCCTGAGATTTGAAGACACGGCCGTCTATTA25130.1278192849952977No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG25040.12736151596825523No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA24800.12614079856280871No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA24570.12497094438258911No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24360.1239028166528234No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG24350.12385195342759646No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA24100.12258037279692298No Hit
AGTCAGGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAA24100.12258037279692298No Hit
GGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAAAGCCC23940.12176656119329196No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC23840.12125792894102257No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG23760.12085102313920705No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23740.12074929668875317No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC23630.12018980121125684No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTA23390.11896908380581031No Hit
ATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCA23270.11835872510308704No Hit
GTAGTAAGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA22120.11250945420198906No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC22000.11189909549926579No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC21890.11133960002176946No Hit
GCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCT21400.10884730198564944No Hit
CCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCCTGG21330.10849125940906088No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC21170.10767744780542986No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21110.10737226845406822No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA20940.10650759362521026No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA20900.1063041407243025No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA20870.10615155104862167No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC20750.10554119234589843No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGG20550.10452392784135964No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20440.1039644323638633No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCC20440.1039644323638633No Hit
TGAGAAGACATTCCCCTCCTGCCACCTGCTCTTGTCCACGGTTAGCCTGC20380.10365925301250169No Hit
GTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCCCCTGGCCGAAAGTG20070.10208249303046656No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG20030.1018790401295588No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA19830.10086177562502004No Hit
CCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGT19820.10081091239979309No Hit
CCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCA19810.10076004917456616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)