FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417950
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC165280.483564709840694No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT161910.4737049986102781No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA121820.3564124694626896No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT105990.31009815825275383No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC98990.28961804590470897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC98000.2867215728726283No Hit
ATCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTAAAGGTCTCCTGCA95210.27855878523676475No Hit
ATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTAGA91510.2677335829956553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC87240.25524071446334795No Hit
GAGTTATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCA84720.24786787401805174No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC82290.24075834930294476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT72580.21234950774587105No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT71620.20954080662385347No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA70080.20503518190728362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG69200.2024605392121008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT66970.1959361605640808No Hit
GGTATCAGCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCA66520.19461958191313505No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT64820.1896458403428956No Hit
ACTTACTACTGTCAACAGAGTTATGGTATCCCGAACACTTTCGGCCAGGG63450.18563758978334968No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG62080.18162933922380375No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATAAGTAA60930.1782647493380535No Hit
GTCTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGG60130.1759241650697055No Hit
CTCCTATCTTTGGTTTTGCAAACTACGCGCAGAACTTCCAGGGCAGAGTC59270.17340803698123144No Hit
CTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTT58480.1710967100162378No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58050.16983864597200077No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56710.16591816732251788No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG56450.1651574774353048No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC55150.1613540279992393No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG54700.16003744934829356No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA53950.15784315159671733No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC53640.15693617519273248No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT53060.1552392515981802No Hit
ATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCAAACGA52310.15304495384660396No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT51740.15137728755540603No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC51630.1510554572185082No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG51260.14997293699439723No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG50910.14894893137699497No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT50480.14769086733275794No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG49980.14622800216504045No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC49800.14570137070466216No Hit
CCATAACTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGTTGCAGACT49210.14397518980675553No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG49070.14356558755979462No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49030.1434485583463772No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG48960.14324375722289678No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA48260.14119574598809229No Hit
CTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGGCC48190.14099094486461183No Hit
TTCAGATACTTTGCGTTCAACTGGGTGCGACAGGTCCCTGGACAAGGGCT48150.14087391565119442No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC47730.13964510891031173No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG47270.13829927295601166No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG46400.13575388756418322No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG46380.13569537295747452No Hit
ATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCA46060.13475913925013533No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC46040.1347006246434266No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA45250.13238929767843297No Hit
GTCTACTTGGAATTGAGCAGCCTGAGATTTGAAGACACGGCCGTCTATTA45240.13236004037507865No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA45210.13227226846501558No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC45100.13195043812811774No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTA44890.13133603475767638No Hit
GCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCT44880.13130677745432204No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA44540.13031202914027415No Hit
CAATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTA44250.129463567342998No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG43850.128293275208824No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG43290.1266548662209804No Hit
AGTCAGGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAA42930.1256016033002238No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC42330.12384616509896282No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG41550.12156409543732355No Hit
CCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCCTGG40630.11887242352872336No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40600.1187846516186603No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40530.11857985049517986No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC40110.11735104375429717No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC40020.11708772802410802No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA39770.11635629544024928No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39750.11629778083354057No Hit
GTAGTAAGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA39150.11454234263227958No Hit
GGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAAAGCCC39120.11445457072221653No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCC39000.11410348308196433No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC38850.11366462353164909No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC38440.11246507409412075No Hit
GTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCCCCTGGCCGAAAGTG38140.11158735499349026No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGG37790.110563349376088No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG37510.10974414488216622No Hit
CCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCA37470.1096271156687488No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTACTACTGTCAAC37300.10912974151172487No Hit
CTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTTCAGTGGCAGT37190.10880791117482702No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA37050.10839830892786613No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA36710.10740356061381823No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA36300.10620401117628989No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC36060.10550183589578548No Hit
TGAGAAGACATTCCCCTCCTGCCACCTGCTCTTGTCCACGGTTAGCCTGC35980.10526777746895069No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT35850.10488743252534413No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC35820.10479966061528109No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA35790.10471188870521805No Hit
GTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACA35640.10427302915490279No Hit
AGATAGGAGTGATCCCTCCCATGCACTCAAGCCCTTGTCCAGGGACCTGT35240.1031027370207288No Hit
CCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGT34890.10207873140332656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)