FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138240
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA92950.4347033073929961No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT86370.4039303352289734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73450.3435068093385214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66770.31226616282550135No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGCCTGCAGGCTAC58180.2720929362466327No Hit
TAATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAG55150.25792240347201434No Hit
CAATATAATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT54050.252777985633044No Hit
GTATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGCCTGCAGGCT52740.24665145166117927No Hit
GTATTAGTACCTGGCTGGCCTGGTATCACCAGAAACCAGGGAAAGCCCCT50680.23701736007183477No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC48360.2261673151750973No Hit
GTATTTATTACGTCCATCATATGAAATCATTGCCACCCACTCCAGCCCCT46880.21924573480993714No Hit
TCTCTAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGC40460.18922104160431008No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38670.1808496707572583No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37900.17724857826997906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36430.17037376533971865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT36410.1702802304699192No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35580.16639853337324154No Hit
ATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA35470.1658840915893445No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33870.1584013020053876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33760.15788686022149057No Hit
CTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT33440.1563903023046992No Hit
AATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA32950.1540986979946124No Hit
GAGTATTAGTACCTGGCTGGCCTGGTATCACCAGAAACCAGGGAAAGCCC32440.15171355881472615No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31900.14918811733014067No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA31350.1466159084106555No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30840.14423076923076925No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACT29900.13983463035019456No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29630.13857190960790183No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29580.13833807243340318No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA29020.13571909607901825No Hit
GTAAAAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA28950.13539172403472013No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC28550.13352102663873092No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG28220.1319777012870398No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA28050.13118265489374437No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27310.1277218647111643No Hit
ACTTATTACTGCCAACAATATAATACTCTAGAGCTCACTTTCGGCGGAGG26940.12599146961987429No Hit
TCAGTAGCCTGCAGGCTGATGATGTTGCAACTTATTACTGCCAACAATAT26370.12332572583058964No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG26200.12253067943729422No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26140.12225007482789584No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25890.12108088895540257No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC24760.11579616881173302No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24280.11355133193654596No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC24050.11247568093385213No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23790.11125972762645914No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23130.10817307692307693No Hit
ATAATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA22970.10742479796468124No Hit
GAATTCACTCTCACCATCAGTAGCCTGCAGGCTGATGATGTTGCAACTTA22950.10733126309488178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22520.10532026339419336No Hit
CTAGAGTATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGCCTGC22300.10429137982639929No Hit
TTTGAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG21790.10190624064651303No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTAC21780.1018594732116133No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21690.10143856629751573No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT21630.10115796168811735No Hit
CCTTAGAGATCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGGTGATACCAG21480.10045645016462136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)