FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005112107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4021524
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94770.23565693005935065No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT70240.17466015371287105No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG64370.16006369724512398No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC64070.1593177113949836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC52580.13074645333460647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49060.12199355269295918No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT45930.1142104336564944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)