FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052649
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.2523568325612416No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT47200.22994676634923947No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT29560.14400903417973557RNA PCR Primer, Index 47 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.12213486085541171No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC24860.12111179261529857No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT23430.11414518507548051RNA PCR Primer, Index 47 (95% over 24bp)
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT22730.11073495760843671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22700.11058880500270625No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG22480.10951701922734963No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA21220.1033786097866708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)